Juglone exerts antitumor effect in ovarian cancer cells

نویسندگان

  • Duo Zhang Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Fang Fang Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Liangzhong Zhao Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Liguo Wang Department of Urology Surgery, Affiliated Hospital of Jilin Medical College, Jilin, Jilin, China
  • Ling Qi Department of Pathology, Jilin Medical College, Jilin, Jilin, China
  • Qiang Li Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Qing Fang Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Shuang Chen Department of Immunology, Jilin Medical College, Jilin, Jilin, China
  • Yingxin Qin Department of Narcotization, Affiliated Hospital of Jilin Medical College, Jilin, Jilin, China
چکیده مقاله:

Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-treated SKOV3 cells. To investigate molecular mechanism of cell cycle and apoptosis, protein expression levels were measured by Western blot analysis of cyclin D1, Bcl-2, Bax, cytochrome c, caspase-9 and caspase-3. To investigate the motility of juglone-treated SKOV3 cell, Matrigel invasion assay was employed to characterize cell invasion. Also, matrix metalloproteinase-2 (MMP-2) expression levels were detected by western blot. Results:Juglone significantly inhibited SKOV3 cell proliferation as shown by G0/G1 phase arrest, and this effect was mediated by inactivation of cyclin D1 protein (P

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juglone exerts antitumor effect in ovarian cancer cells

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عنوان ژورنال

دوره 18  شماره 6

صفحات  544- 548

تاریخ انتشار 2015-06-01

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